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Prevalence and significance of anti-saccharomyces cerevisiae antibodies in primary Sjögren’s syndrome

1, 2, 3, 4, 5, 6, 7, 8

  1. Rheumatology Unit, Department of Medicine, University of Perugia, Italy.
  2. Rheumatology Unit, Department of Medicine, University of Perugia, Italy.
  3. Rheumatology Unit, Department of Biotechnological and Applied Clinical Science, School of Medicine, University of L'Aquila; and Department of Medicine, ASL1 Avezzano-Sulmona-L'Aquila, Italy.
  4. Rheumatology Unit, Department of Medicine, University of Perugia, Italy.
  5. Rheumatology Unit, Department of Medicine, University of Perugia, Italy.
  6. Rheumatology Unit, Department of Medicine, University of Perugia, Italy.
  7. Rheumatology Unit, Department of Biotechnological and Applied Clinical Science, School of Medicine, University of L'Aquila, Italy.
  8. Rheumatology Unit, Department of Medicine, University of Perugia, Italy. roberto.gerli@unipg.it

CER10350 Submission on line
2018 Vol.36, N°3 ,Suppl.112 - PI 0073, PF 0079
Clinical aspects

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Rheumatology Article

 

Abstract

OBJECTIVES:
Saccharomyces cerevisiae is a common yeast used in the food industry. IgG and IgA antibodies against the phosphopeptidomannan of the S. cerevisiae cell wall (ASCA) are a well known marker of disease severity in Crohn’s disease. Moreover, a number of studies assessed ASCA in several systemic and organ-specific autoimmune diseases postulating molecular mimicry as a possible link between ASCA and autoimmunity. However, since they have never been tested in primary Sjögren’s syndrome (pSS), the purpose of this study was to investigate these antibodies in a large cohort of pSS patients, compared to healthy donors (HD), and their significance as potentially helpful biomarker in a clinical setting.
METHODS:
ASCA IgG+IgA were assessed with ASCA screen dot for Blue Diver instrument (Alphadia sa/nv, Belgium). The comparison between the aminoacid sequence of mannan of S. cerevisiae and well characterised auto-antigens peculiar to pSS (52kD and 60kD Ro/SSA, La/SSB) was performed with the Basic Local Alignment Search Tool (BLAST).
RESULTS:
The prevalence of ASCA in our pSS cohort was 4.8%. We also reported that the ASCA target protein has a high similarity with Ro60/SSA protein further supporting the molecular mimicry hypothesis. Finally, we observed that ASCA positivity is associated with pSS specific clinical and serological features. ASCA+ pSS patients displayed a triple combination of circulating anti-Ro52/SSA, anti-Ro60/SSA and anti-La/SSB antibodies, associated with low complement and cutaneous involvement.
CONCLUSIONS:
Our data suggest a possible pathogenic/prognostic significance of ASCA in pSS.

PMID: 28664835 [PubMed]

Received: 21/02/2017 - Accepted : 03/04/2017 - In Press: 29/06/2017 - Published: 13/08/2018