ab226012

Anti-inflammatory effect of mud-bath applications on adjuvant arthritis in rats

F. Cozzi¹, M. Carrara², P. Sfriso¹, S. Todesco¹, L. Cima²

¹Division of Rheumatology, Department of Medical and Surgical Sciences; ²Department of Pharmacology and Anaesthesiology, University of Padova, Italy.

ABSTRACT
Objective
The real effects of mud-bath applications on the inflammatory process are still not clarified. We studied these effects on rat adjuvant-induced arthritis.

Methods
Arthritis was induced in 30 rats by subplantar injection of Freund's complete adjuvant (FCA) into the right hind paw. Ten days after FCA injection, the rats were randomized in 3 groups of 10 each: the first one was submitted to a cycle of mud-bath applications, the second one was treated with indomethacin, the third one received only saline per os (control group). The paw volume, measured by plethysmometry, and the serum levels of TNFa and IL-1b were considered as evaluation parameters.

Results
FCA injection caused a progressive enhancement of paw volume and a rapid increase of TNFa and IL-1b serum levels. After the randomization, mud-bath applications reduced inflammation and at the end of the treatment the paw volume and the TNFa and IL-1b serum levels were significantly tapered in comparison to the controls (p < 0.01).

Conclusion
The results of the study suggest an anti-inflammatory effect of mud-bath applications on adjuvant arthritis in rats. These results could explain the beneficial effects of thermal treatments observed in some inflammatory rheumatic diseases.

Key words
Adjuvant arthritis, TNFa, IL-1b, mud bath, anti-inflammatory agents

Please address correspondence and reprint requests to: Franco Cozzi, MD, Division of Rheumatology, Department of Medical and Surgical Sciences, University of Padova, Via Giustiniani 2, 35128 Padova, Italy.
E-mail: franco.cozzi@unipd.it