Aetiopathogenesis
Increased expression of interferon-λ in minor salivary glands of patients with primary Sjögren's syndrome and its synergic effect with interferon-α on salivary gland epithelial cells
Y.-J. Ha1, Y.S. Choi2, E.H. Kang3, J.-H. Chung4, S. Cha5, Y.W. Song6, Y.J. Lee7
- Division of Rheumatology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
- Division of Rheumatology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
- Division of Rheumatology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
- Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea.
- Departments of Oral and Maxillofacial Diagnostic Sciences and Oral Biology, University of Florida College of Dentistry, Gainesville, USA.
- Department of Internal Medicine, Seoul National University Hospital; and WCU Department of Molecular Medicine and Biopharmaceutical Sciences, Medical Research Institute, Seoul, Korea.
- Division of Rheumatology, Department of Internal Medicine, Seoul National University Bundang Hospital; and Department of Internal Medicine, Seoul National University College of Medicine, Korea. yn35@snu.ac.kr
CER10168
2018 Vol.36, N°3 ,Suppl.112
PI 0031, PF 0040
Aetiopathogenesis
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PMID: 28421993 [PubMed]
Received: 11/12/2016
Accepted : 06/03/2017
In Press: 18/04/2017
Published: 13/08/2018
Abstract
OBJECTIVES:
To investigate the expressions of interferon (IFN)-λs and their receptor, IL28RA, in minor salivary glands (MSG) of pSS patients and their effects on the salivary gland cells.
METHODS:
The expressions of IFN-λs and IL28RA were evaluated in MSG by immunohistochemistry in 15 patients with pSS and in 5 patients with non-SS sicca. Poly(I:C)-induced IL-28A and IL-29 expressions were determined in immortalized human salivary gland acinar (NS-SV-AC) and ductal (NS-SV-DC) cell lines. We assessed the effect of IFN-λs on the expressions of typical interferon-inducible genes, B-cell activating factor (BAFF) and CXCL10, and the synergistic effect of IL-29 and type I or II IFN on their expressions. The serum IL-29 levels were measured in 44 patients with pSS and 22 healthy controls.
RESULTS:
IFN-λs expression was significantly higher in MSG from pSS than from non-SS sicca controls. Poly(I:C) treatment led to the induction of IL-28A and IL-29 in the salivary gland cell lines. In the NS-SV-DC cells, IFN-λ significantly increased the levels of BAFF and CXCL10 in a time and dose-dependent manner. Moreover, there was a synergistic effect between IL-29 and IFN-α in the induction of BAFF and CXCL10 expressions by prolonged STAT1 phosphorylation. However, the serum IL-29 levels were not significantly higher in pSS patients than in healthy controls.
CONCLUSIONS:
Our results suggest the possibility for IFN-λ to play a role by participating local inflammation in the salivary glands of pSS through direct and indirect regulations of the expressions of BAFF and CXCL10 in salivary gland epithelium.
