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Serum Epstein-Barr virus DNA, detected by droplet digital PCR, correlates with disease activity in patients with rheumatoid arthritis
E. Kuusela1, V.-P. Kouri2, J. Olkkonen3, R. Koivuniemi4, L. Äyräväinen5, K. Rajamäki6, H. Valleala7, D. Nordström8, M. Leirisalo-Repo9, M. Ainola10, K.K. Eklund11
- University of Helsinki, Clinicum, Helsinki, Finland. elina.kuusela@helsinki.fi
- University of Helsinki, Clinicum, Helsinki, Finland.
- University of Helsinki, Clinicum, Helsinki, Finland.
- Department of Rheumatology, Helsinki University and Helsinki University Hospital, Finland.
- Department of Oral and Maxillofacial Diseases, University of Helsinki, Finland.
- University of Helsinki, Clinicum, Helsinki, Finland.
- Department of Rheumatology, Helsinki University and Helsinki University Hospital, Finland.
- Internal Medicine and Rehabilitation, Helsinki University and Helsinki University Hospital, Finland.
- Department of Rheumatology, Helsinki University and Helsinki University Hospital, Finland.
- University of Helsinki, Clinicum, Helsinki, Finland.
- Department of Rheumatology, Helsinki University and Helsinki University Hospital, and ORTON Orthopaedic Hospital, Helsinki, Finland.
CER10686
2018 Vol.36, N°5
PI 0778, PF 0784
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PMID: 29600942 [PubMed]
Received: 13/07/2017
Accepted : 18/12/2017
In Press: 20/03/2018
Published: 26/09/2018
Abstract
OBJECTIVES:
To study the prevalence of asymptomatic activation of Epstein-Barr virus (EBV) in patients with rheumatoid arthritis (RA) and to analyse the correlation of serum EBV DNA with the disease activity.
METHODS:
The level of EBV DNA was determined by droplet digital PCR assay from the serum of 46 DMARD naive early RA (ERA) and 22 chronic RA (CRA)-patients at study onset. Follow-up samples from 31 ERA and 16 CRA patients were obtained after starting or modifying the anti-rheumatic treatment. EBV DNA was also measured from 33 healthy controls and 9 patients with adult onset Still’s disease (AOSD). Disease activity was assessed by the disease activity score (DAS28).
RESULTS:
At baseline, EBV DNA was detected in the serum of 7 of the 46 ERA patients all of whom had moderate or high disease activity. In the follow-up samples, 11 of 31 patients were EBV DNA positive. At baseline EBV positive patients had significantly higher disease activity (p=0.036) and the concentration of EBV DNA correlated significantly with DAS28 (rs=0.333, p=0.024). EBV DNA was detected in 3 of 22 CRA patients at study onset and in 8 of 16 in the follow-up samples. At follow-up EBV positive patients had significantly higher DAS28 (p=0.027) and the concentration of EBV DNA correlated significantly with DAS28 (rs=0.724, p=0.002). Only one of the healthy controls and none of the AOSD patients were positive for EBV DNA.
CONCLUSIONS:
Active RA is associated with a lytic EBV infection which may have a role in the pathogenesis of RA.