Rapid paper
Involvement of pentraxin-3 in anti-neutrophil cytoplasmic antibody production induced by aluminum salt adjuvant
K. Nagai1, Y. Aratani2, A. Shibuya3, K. Yamagata4
- Department of Nephrology, Faculty of Medicine, University of Tsukuba, Japan. knagai@md.tsukuba.ac.jp
- Department of Life and Environmental System Science Graduate School of Nanobioscience, Yokohama City University, Japan.
- Department of Immunology, Faculty of Medicine, University of Tsukuba, Japan.
- Department of Nephrology, Faculty of Medicine, University of Tsukuba, Japan.
CER10716
2017 Vol.35, N°5
PI 0735, PF 0738
Rapid paper
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PMID: 28850023 [PubMed]
Received: 28/07/2017
Accepted : 17/08/2017
In Press: 28/08/2017
Published: 13/09/2017
Abstract
OBJECTIVES:
Pentraxin 3 (PTX3) is a multifunctional soluble factor. PTX3 can be involved in the regulation of vasculitis and is expressed in the cytoplasm of neutrophils. As anti-neutrophil cytoplasmic antibody (ANCA) is recognised as a cause of vasculitis, we aimed to discover the role of PTX3 in ANCA production in vivo.
METHODS:
To this end, we used aluminum salt (alum), which induces neutrophil extracellular traps, as an adjuvant for producing anti-myeloperoxidase-ANCA (MPO-ANCA). Specifically, we intraperitoneally injected alum and recombinant MPO (rMPO) into MPO-deficient mice and then measured the concentration of anti-MPO IgG in their blood. To show the involvement of extracellular PTX3 in this model, we assessed PTX3 protein content and host double-stranded DNA levels in the mice’s peritoneal fluid after alum injection. In addition, we simultaneously administered recombinant PTX3, rMPO and alum to MPO-deficient mice to assess the function of PTX3 in producing anti-MPO IgG in vivo.
RESULTS:
Anti-MPO IgG was produced by the alum + rMPO immunisation model in MPO-deficient but not wildtype mice. Injection of alum induced extracellular PTX3 as well as double-stranded DNA and dead cells in MPO-deficient mice. Simultaneous injection of recombinant PTX3 with rMPO and alum attenuated the production of anti-MPO IgG in MPO-deficient mice.
CONCLUSIONS:
Our current findings provide evidence that PTX3 attenuates the production of murine MPO-ANCA.
