Full Papers
The expression characteristics of cytochrome C oxidase subunit I in mitochondrial of MRL/lpr lupus mice
S. Zhang1, Y. Deng2, X. Huang3, N. Li4, H. Fan5, B. Xiang6, Y. Wu7, X. Cheng8, X. Chen9
- Department of Dermatology, The First Affiliated Hospital of Guangzhou Medical University, China.
- Department of Dermatology, The First Affiliated Hospital of Guangzhou Medical University, China.
- Department of Dermatology, The First Affiliated Hospital of Guangzhou Medical University, China.
- Department of Dermatology, The First Affiliated Hospital of Sun Yat-sen University, China.
- Department of Dermatology, The Affiliated Hospital of Guizhou Medical University, China.
- Department of Laboratory, The First Affiliated Hospital of Guangzhou Medical University, China.
- Department of Dermatology, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, China.
- Department of Dermatology, The First Affiliated Hospital of Guangzhou Medical University, China. cxpsyggyx@126.com
- Department of Dermatology, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, China. xinshengchen@sohu.com
CER12953
2021 Vol.39, N°1
PI 0044, PF 0051
Full Papers
PMID: 32242809 [PubMed]
Received: 16/11/2019
Accepted : 10/02/2020
In Press: 28/03/2020
Published: 05/02/2021
Abstract
OBJECTIVES:
We sought to analyse the expression characteristics of cytochrome C oxidase subunit I in mitochondrial of MRL/lpr lupus mice.
METHODS:
The whole blood of MRL/lpr lupus mice was detected for whole mitochondrial genome sequencing performed by Illumina HiSeq PE150 instrument, compared with house mouse (NC_005089.1) and screened for the maximum difference gene, MT-CO1. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were used to detect the mRNA and protein expression of MT-CO1 in lupus mice and control mice. The total antioxidant capacities of lupus mice and control mice were measured using the rapid 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) method.
RESULTS:
The mitochondrial genome sequencing showed that five mitochondrial genes had base differences and MT-CO1 was the maximum difference gene, 31 in total. Among the 31 base difference sites, 2 were missense mutations and 29 were synonymous_variant. qRT-PCR test results showed that the MT-CO1 expression in lupus mouse blood was statistically lower than that in control mice blood (t=4.333; p=0.0003). Western blot test results revealed that the expression of MT-CO1 was lower in the lupus mice compared with the control mice at the protein level. Serum total antioxidant capacity testing showed that: the serum total antioxidant capacity of lupus mice was statistically lower than that of the control mice (t=9.957; p<0.0001).
CONCLUSIONS:
High mutation rate and decreased expression of MT-CO1 in MRL/lpr lupus mice accompanied the decrease of antioxidant capacity, which indicated that abnormal MT-CO1 might be involved in the pathogenesis of SLE and the production of anti-dsDNA antibodies.
