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Aetiopathogenesis

 

Acinar adipose tissue infiltration in salivary gland biopsy is associated with kynurenines-interferon-γ pathway inflammation biomarkers


1, 2, 3, 4, 5, 6, 7, 8, 9, 10

 

  1. Hospital Universitário Cassiano Antônio de Moraes, Departamento de Clínica Médica, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, Brazil.
  2. Hospital Universitário Cassiano Antônio de Moraes, Departamento de Clínica Médica, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, Brazil.
  3. Gade Laboratorium for Pathology, Department for Clinical Medicine, University of Bergen, Haukeland University Hospital, Bergen, Norway.
  4. Hospital Universitário Cassiano Antônio de Moraes, Departamento de Clínica Médica, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, Brazil.
  5. Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.
  6. Section for Pharmacology, University of Bergen, Norway.
  7. Section for Pharmacology, University of Bergen, Norway.
  8. Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.
  9. Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.
  10. Hospital Universitário Cassiano Antônio de Moraes, Departamento de Clínica Médica, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, Brazil. val.valim@gmail.com

CER13138
2020 Vol.38, N°4 ,Suppl.126
PI 0027, PF 0033
Aetiopathogenesis

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PMID: 33095140 [PubMed]

Received: 24/01/2020
Accepted : 22/06/2020
In Press: 22/10/2020
Published: 22/10/2020

Abstract

OBJECTIVES:
Assess if kynurenines metabolites are biomarkers of damage at labial salivary gland biopsy (LSGB).
METHODS:
This is a cross-sectional study including 99 patients with primary Sjögren’s syndrome (AECG 2002 or ACR/EULAR 2017). Kynurenines were measured in plasma using liquid chromatography-tandem mass spectrometry.
RESULTS:
95.9% were females, 51±12 years. Most had focal lymphocytic sialadenitis with focus score ≥1 (73.7%, n=73/99). The majority had mild to severe acinar atrophy (70.4%, n=57/81) and adipose infiltration (51.2%, n=39/80). Individuals with adipose infiltration were older (53.49±12.33 vs. 47.51±11.29 years, p=0.016), showed higher frequency of glandular dysfunction and higher kynurenines levels. Schirmer’s test ≤ 5 mm/5min was found in 69.2% of individuals with adipose infiltration compared to 41% without (p=0.012) and unstimulated whole salivary flow (UWSF) was found in 87.2% compared to 70% without adipose infiltration (p=0.063). Additionally, individuals with adipose infiltration showed higher kynurenines metabolites compared with those without: quinolinic acid (503.35±193.30 vs. 427.35±285.76 nmol/L, p=0.029), kynurenine (1.99±0.6, 54 vs. 1.61±0.46 μmol/L, p=0.006), kynurenine/tryptophan ratio (KTR) (0.030±0.09 vs. 0.025±0.01, p=0.031) and anthranilic acid (03±4.96 vs. 16.46±5.24 nmol/L, p=0.022).
CONCLUSIONS:
Kynurenines are biomarkers of greater adipose infiltration in LSGB and glandular dysfunction suggesting that activation of interferon-γ pathway is involved in the salivary and lacrimal glands damage.

Rheumatology Article