impact factor, citescore
logo
In press
In Press
Recent issue
Recent issue
Recent Supplement
Recent supplement
archives
Archives
articles free
Free to view
Search
Search
 

Full Papers

 

Alteration of oral flora in Mongolian patients with Behçet’s disease: a multicentre study

1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16

 

  1. Department of Ophthalmology, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, and Glaucoma Clinic, Zalaa Khukh Tolgoi LLC, Ulaanbaatar, Mongolia.
  2. Health Sciences University of Hokkaido School of Dentistry, Tobetsu, Hokkaido, Japan.
  3. Health Sciences University of Hokkaido School of Dentistry, Tobetsu, Hokkaido, Japan.
  4. Department of Ophthalmology, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
  5. Department of Molecular Biology and Genetics, School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
  6. Health Sciences University of Hokkaido School of Dentistry, Tobetsu, Hokkaido, Japan.
  7. Health Sciences University of Hokkaido School of Dentistry, Tobetsu, Hokkaido, Japan.
  8. Immunoregulation Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
  9. Health Sciences University of Hokkaido School of Dentistry, Tobetsu, Hokkaido, Japan.
  10. Schepens Eye Research Institute, Harvard Medical School, Boston, MA, USA.
  11. Advanced Research Promotion Center, Health Sciences University of Hokkaido, Tobetsu, Hokkaido, Japan.
  12. Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo and Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  13. Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  14. Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  15. Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo and Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  16. Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo and Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan. nobukita@hoku-iryo-u.ac.jp

CER13979
2020 Vol.38, N°5 ,Suppl.127
PI 0080, PF 0085
Full Papers

Free to view
(click on article PDF icon to read the article)

PMID: 33331270 [PubMed]

Received: 29/08/2020
Accepted : 17/11/2020
In Press: 10/12/2020
Published: 10/12/2020

Abstract

OBJECTIVES:
Behçet’s disease (BD) is characterised by repeated acute inflammatory attacks with aphthous ulcers of the oral mucosa, uveitis of the eyes, skin symptoms, and genital ulcers. Although its aetiology is still unknown, there is evidence of the involvement of oral bacteria in systemic diseases. Various types of oral bacteria may be involved in the development and progression of BD. The present study investigated alterations in the oral flora of patients with BD in Mongolia. We collected saliva samples from the Mongolian BD group and healthy control (HC) group, and the oral flora were analysed using next-generation sequencer (NGS).
METHODS:
DNA was extracted from the unstimulated saliva samples from the 47 BD and 48 HC subjects. The DNA was amplified from the V3–V4 region of 16S rRNA using PCR, and the data were acquired using NGS. Based on the obtained data, we analysed the alpha diversity, beta diversity, and bacterial taxonomy of the salivary flora.
RESULTS:
Beta diversity differed significantly between the BD and HC flora, but no significant differences were observed in alpha diversity. We found that the proportions of three genera – an S24-7 family unknown species, a mitochondria family unknown species, and Akkermansia species associated with IL-10 production – were significantly lower in the BD than in the HC group.
CONCLUSIONS:
The reduced proportions of the S24-7 family and symbiotic Akkermansia species may be key phenomena in the oral flora of patients with BD.

Rheumatology Article