Full Papers
Plasma micro-RNA-22 is associated with disease activity in well-established rheumatoid arthritis
M. Cieśla1, B. Kolarz2, M. Majdan3, D. Darmochwał-Kolarz4
- College of Medical Sciences, University of Rzeszow, Poland. ciesla_marek@wp.pl
- College of Medical Sciences, University of Rzeszow, Poland.
- Department of Rheumatology and Connective Tissue Diseases, Medical University of Lublin, Poland.
- College of Medical Sciences, University of Rzeszow, Poland.
CER14454
2022 Vol.40, N°5
PI 0945, PF 0951
Full Papers
Free to view
(click on article PDF icon to read the article)
PMID: 34369363 [PubMed]
Received: 22/01/2021
Accepted : 26/04/2021
In Press: 24/07/2021
Published: 13/05/2022
Abstract
OBJECTIVES:
Micro-RNAs (miRNAs) are an endogenous small, single-stranded, non-coding RNAs with a 18-25 nucleotide long and have been reported as potential extracellular biomarkers of various diseases. They mainly decrease the gene expression by inhibiting the translation or cause mRNA destabilisation. The aim of our study was to identify miRNAs whose concentration may be associated with severity of rheumatoid arthritis (RA).
METHODS:
A total of 74 unrelated individuals, 50 with RA and 24 in a control group were enrolled to the study. Real-time PCR was used to evaluate the plasma concentration levels of 8 miRNAs: miR-26a, miR-125b, miR-20b, miR-22, miR-221, miR-17, miR-93, miR-106b.
RESULTS:
The logistic regression results showed that miR-22 (p=0.0003) and miR-26a (p=0.049) may be the most important molecules distinguishing RA patients and healthy controls. Moreover, the quantity of miR-22 was different between rheumatoid factor (RF)-positive and RF-negative patients (p=0.04).
CONCLUSIONS:
In this study we demonstrated for the first time that plasma concentration of miR-22 may be considered as a potential molecular marker associated with disease activity.
