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Alterations of peripheral blood B cell subsets in Chinese patients with adult idiopathic inflammatory myopathies


1, 2, 3, 4, 5, 6, 7, 8, 9, 10

 

  1. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  2. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  3. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  4. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  5. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  6. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  7. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  8. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  9. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China.
  10. Department of Rheumatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi Province, China. xajdhl87@mail.xjtu.edu.cn

CER14603
2022 Vol.40, N°2
PI 0260, PF 0266
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PMID: 34905483 [PubMed]

Received: 07/03/2021
Accepted : 28/06/2021
In Press: 10/12/2021
Published: 25/02/2022

Abstract

OBJECTIVES:
Abnormalities and hyperactivation of B cells have been described in idiopathic inflammatory myopathies (IIM). However, little is known about changes in the homeostasis of peripheral blood B cells in adult IIM patients. The aim of this study was to identify phenotypic alterations of B cell subsets and their relation to the overall clinical profile.
METHODS:
Blood samples were collected from 25 adult IIM patients and 15 healthy controls. Peripheral B cell subsets were classified into non-switched memory B cells (CD19+CD27+lgD+), switched memory B cells (CD19+CD27+lgD−), double-negative (DN) memory B cells (CD19+CD27−lgD−) and naïve B cells (CD19+CD27−lgD+) based on their surface phenotype as measured by flow cytometry. The clinical profile of IIM and its correlation with B cell subsets was further evaluated.
RESULTS:
Frequencies of CD19+ B cells and naïve B cells were increased in adult IIM patients compared with healthy controls (p=0.005 and p<0.001, respectively) and the frequency of memory B cells was decreased (p<0.001). Moreover, patients with a rash had lower non-switched memory B cells proportion (p=0.032). Patients with anti-MDA5+ antibodies had higher CD19+ B cells proportion than anti-ARS+ patients (p=0.046). Patients who were not receiving treatment had elevated levels of CD19+ B cells and naïve B cells along with reduced non-switched memory B cells compared with patients who were receiving treatment (p=0.021, p=0.036 and p=0.032, respectively).
CONCLUSIONS:
Our findings demonstrate abnormalities in the homeostasis of the B cell subsets present in adult IIM patients, characterised by expanded CD19+ B cells and naïve B cells but reduced memory B cells. Phenotypic abnormalities of B cell subsets are associated with the presence of a rash, with anti-MDA5 positivity and with treatment.

DOI: https://doi.org/10.55563/clinexprheumatol/ohsmuj

Rheumatology Article