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The expression of Siglec-10 on naive B cells is involved in the pathology of systemic lupus erythematosus
B. Ju1, J. Luo2, N. Hu3, J. Zhang4, L. Zhu5, Q. Li6, Y. Wang7, J. Huang8, Q. An9, Q. Xu10, Z. Hao11, D. Pu12, X. Lv13, X. Li14, Y. Huo15, B. Zhang16, L. He17
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China.
- Department of Human Anatomy and Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi’an, Shaanxi, China. hyw301@xjtu.edu.cn
- Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an, Shaanxi; and Institute of Infection and Immunity, Translational Medicine Institute, Xi’an Jiaotong University Health Science Center, Xi’an, Shaanxi, China. bj.zhang@mail.xjtu.edu.cn
- Department of Rheumatology and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi, China. xajdhl87@mail.xjtu.edu.cn
CER17941
2025 Vol.43, N°3
PI 0507, PF 0516
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PMID: 39711368 [PubMed]
Received: 24/06/2024
Accepted : 20/11/2024
In Press: 21/12/2024
Published: 12/03/2025
Abstract
OBJECTIVES:
Previous studies have reported the expansion of CD19+Siglec-10+ B cells in systemic lupus erythematosus (SLE) patients. However, the composition of this cell population, phenotype and characteristics are still unknown.
METHODS:
We examined this memory B-cell subset’s composition and phenotype and determined the SYK and AKT phosphorylation levels by flow cytometry. Additionally, we explored the relationship between Siglec-10 expression on B-cell subsets and clinical manifestations.
RESULTS:
Our results indicated elevated levels of Siglec-10 on naive B cells in active SLE patients. Compared with healthy controls (HCs) and inactive SLE patients, the Siglec-10+ B cells in active SLE patients exhibited elevated CD40 and CD21low levels. The levels of Siglec-10 on naive B cells were positively correlated with the proportion of CD21low double negative (DN) B cells and the SLEDAI-2K score.
CONCLUSIONS:
The results indicate that the upregulation of Siglec-10+/naive B cells may function as a feedback mechanism to regulate B cell hyperreactivity. Monitoring the proportion of Siglec-10+/naive B cells may contribute to the evaluation of disease progression in SLE.
