Genetic contribution of the CD14 -159C/T dimorphism in the promoter region in Japanese RA

CER3258
2008 Vol.26, N°2
PI 0337, PF 0339
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PMID: 18565258 [PubMed]

Abstract

OBJECTIVES:
To study the contribution of the CD14 gene to the pathogenesis of rheumatoid arthritis (RA) in Japanese patients.
METHODS:
CD14 genotyping was carried out at the -159C/T dimorphic site in 97 RA patients and 104 normal subjects by the PCR-RFLP (restriction fragment length polymorphism)
METHODS:
HLA-DRB1 genotyping was performed by the PCR-SSCP (sequence specific conformational polymorphism) method.
RESULTS:
The -159C/T dimorphism is not associated with whole RA or with female RA, and the results were compatible with a previous report from Germany. The -159C/T dimorphism was not associated with rheumatoid factor (RF)-positive RA, although the -159T allele tended to be associated with RF in the German report. The -159C/T dimorphism showed no association even in RA patients with the RA-susceptibility HLA-DRB1*0405. The -159T allele was prevalent in Japanese controls.
CONCLUSIONS:
The CD14 gene is very unlikely to be genetically involved in the pathogenesis of Japanese RA.