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Microarray analysis of circulating micro RNAs in the serum of patients with polymyositis and dermatomyositis reveals a distinct disease expression profile and is associated with disease activity.


1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12

 

  1. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic. remakova@revma.cz
  2. Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
  3. Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
  4. Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
  5. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  6. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  7. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  8. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  9. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  10. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  11. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
  12. Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.

CER8302
2016 Vol.34, N°1
PI 0017, PF 0024
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PMID: 26574749 [PubMed]

Received: 19/01/2015
Accepted : 17/04/2015
In Press: 17/11/2015
Published: 10/02/2016

Abstract

OBJECTIVES:
The aim of this study was a large scale investigation of myositis-associated circulating miRNA molecules and also determination of expression of these candidate molecules in relation to clinical activity of myositis.
METHODS:
RNA, containing also miRNAs, was isolated from sera of 28 patients suffering from idiopathic inflammatory myopathies (IIM) and 16 healthy controls. Expression of miRNAs was determined using a miRNA microarray method. Statistical analysis of miRNA expression was carried out using Arraystar software.
RESULTS:
Our results showed 23 significantly differentially expressed miRNAs. Six miRNAs were differentially expressed in IIM compared to healthy controls. In dermatomyositis (DM) we found 3 and in polymyositis (PM) 6 differentially expressed miRNAs compared to controls. Three miRNAs were up-regulated in patients with highly active disease compared to patients with low disease activity. Furthermore, we found 26 significantly differentially expressed miRNAs in SLE patients compared to IIM, DM and PM patients.
CONCLUSIONS:
This is the first study that comprehensively describes expression levels of circulating miRNAs in serum of patients suffering from IIM. It can be expected that some of these deregulated miRNA molecules are involved in aetiology of IIM and may potentially serve as molecular markers for IIM development or for monitoring of disease activity.

Rheumatology Article