Special Lecture
Selection of natural autoreactive B cells
R.R. Hardy1, K. Hayakawa2
- Fox Chase Cancer Center, Philadelphia, PA, USA. richard.hardy@fccc.edu
- Fox Chase Cancer Center, Philadelphia, PA, USA.
CER8929
2015 Vol.33, N°4 ,Suppl.92
PI 0080, PF 0086
Special Lecture
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PMID: 26457505 [PubMed]
Received: 03/09/2015
Accepted : 03/09/2015
In Press: 12/10/2015
Published: 14/10/2015
Abstract
Natural antibodies produced by CD5+ B1 B cells include anti-thymocyte autoantibody (ATA). Transgenic mice bearing the Ig-μ heavy chain of a prototypic ATA, VH3609Vκ21c, demonstrated a critical requirement for self-antigen in the accumulation of ATA B cells and production of high levels of serum ATA. Further work with ATA-μκ transgenic mice revealed that, while development of most B cells were blocked at an immature stage in spleen, some mature ATA B cells were present. ATA-μκ transgenic mice with varying levels of Thy-1 autoantigen showed a clear relationship between BCR crosslinking and B cell fate, with low levels generating marginal zone ATA B cells and complete antigen absence allowing maturation to follicular ATA B cells. Finally, different fates of developing ATA B cells encountering high levels self-antigen may be accounted for by variations in the response of newly formed B cells arising from foetal and adult development.