N. Oezguen1, N. Yalcinkaya2, C. Kücükali3, M. Dahdouli4, E. Hollister5, R. Luna6, R. Türkoglu7, M. Kürtüncü8, M. Eraksoy9, T. Savidge10, E. Tüzün11
Altered gut microbiota community dynamics are implicated in diverse human diseases including inflammatory disorders such as neuro-Behçet’s disease (NBD) and multiple sclerosis (MS). Traditionally, microbiota communities are analysed uniformly across control and disease groups, but recent reports of subsample clustering indicate a potential need for analytical stratification. The objectives of this study are to analyse and compare faecal microbiota community signatures of ethno-geographical, age and gender matched adult healthy controls (HC), MS and NBD individuals.
Faecal microbiota community compositions in adult HC (n=14), NBD patients (n=13) and MS (n=13) were analysed by 16S rRNA gene sequencing and standard bioinformatics pipelines. Bipartite networks were then used to identify and re-analyse dominant compositional clusters in respective groups.
We identified Prevotella and Bacteroides dominated subsample clusters in HC, MS, and NBD cohorts. Our study confirmed previous reports that Prevotella is a major dysbiotic target in these diseases. We demonstrate that subsample stratification is required to identify significant disease-associated microbiota community shifts with increased Clostridiales evident in Prevotella-stratified NBD and Bacteroides-stratified MS patients.
Patient cohort stratification may be needed to facilitate identification of common microbiota community shifts for causation testing in disease.
PMID: 31172918 [PubMed]
Received: 25/11/2018 - Accepted : 28/03/2019 - In Press: 30/05/2019