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IL-1β, IL-10 and TNF-α polymorphisms may affect systemic lupus erythematosus risk and phenotype
E. Rzeszotarska1, A. Sowinska2, B. Stypinska3, A. Lutkowska4, A. Felis-Giemza5, M. Olesinska6, M. Puszczewicz7, D. Majewski8, P.P. Jagodzinski9, E. Haładyj10, A. Paradowska-Gorycka11
- Department of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland.
- Department of Computer Science and Statistics, Poznan University of Medical Sciences, Poznan, Poland.
- Department of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland.
- Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland.
- Department of Connective Tissue Diseases, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland.
- Department of Connective Tissue Diseases, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland.
- Department of Rheumatology and Internal Diseases, Poznan University of Medical Science, Poznan, Poland.
- Department of Rheumatology and Internal Diseases, Poznan University of Medical Science, Poznan, Poland.
- Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland.
- Eli Lilly and Company, Indianapolis, IN, USA.
- Department of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland. paradowska_aga@interia.pl
CER14832
2022 Vol.40, N°9
PI 1708, PF 1717
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PMID: 35084314 [PubMed]
Received: 26/05/2021
Accepted : 25/10/2021
In Press: 05/01/2022
Published: 20/09/2022
Abstract
OBJECTIVES:
Systemic lupus erythematosus (SLE) is an autoimmune disease, and IL-1β, IL-10, and TNF-α genes are important in the pathogenesis of this disease. We studied the impact of IL-1β-511, IL-1β +3953, IL-10 -592, IL-10 -1082, TNF-α -308, TNF-α -238, and TNF-α +489 polymorphisms on SLE risk and phenotype in SLE patients and healthy controls.
METHODS:
We genotyped SLE patients and healthy controls by real-time PCR on QuantStudio 5 (Applied Biosystems) and measured levels of cytokines by enzyme-linked immunosorbent assay (ELISA).
RESULTS:
We indicated that TNF-α -308, IL-10 -592, IL-10 -1082, IL-1β-511 and IL-1β +3953 polymorphisms affect SLE risk. Furthermore, we exposed that some of the TNF-α +489, TNF-α -238, IL-10 -1082 and IL-1β +3953 genotypes are connected with the SLE phenotype. Moreover, we discovered the linking between specific genotypes and the serum concentrations of TNF-α, IL-1β, and IL-10.
CONCLUSIONS:
In conclusion, our study revealed that IL-1β-511, IL-1β +3953, IL-10 -592, IL-10 -1082, and TNF-α -308 polymorphisms may affect SLE risk and phenotype.
